Conversion and Calculations

 


Molecular Weights (MW)
 
Average MW RNA nucleotide = 340 Daltons         
MW ss RNA = (# nucleotides) x 340
 
Average MW RNA basepair = 680 Daltons
MW ds RNA = (# nucleotides) x 680
 
Average MW DNA nucleotide = 330 Daltons
MW ss DNA = (# nucleotides) x 330
 
Average MW DNA basepair = 660 Daltons
MW ds DNA = (# basepairs) x 660
 
Average MW of an amino acid = 110 Daltons
Average MW of a protein = (# of amino acids) x 110
 
 
Conversions for Protein and DNA Size and Weight
 
Average molecular weight of an amino acid = 110 Daltons
333 amino acids = 37 kDa protein
10 kDa protein = 270 bp DNA
100 pmol of 1 kDa protein = 100 ng
 
1 kb of ds DNA = 6.6 x 10/5 Daltons
1 kb of ss DNA = 3.3 x 10/5 Daltons
1 kb of ss RNA = 3.4 x 10/5 Daltons
 
Average weight of a nucleotide = 330 Daltons
1 µg of 1000 bp DNA = 1.53 pmol (3.03 pmol of DNA ends)
1 pmol of 1000 bp DNA = 0,66 µg
 
1 A260 unit of ds DNA = 50 µg/ml
1 A260 unit of ss DNA = 33 µg/ml
1 A260 unit of ss RNA = 40 µg/ml