Reconstitution and Storage of Recombinant Proteins
Please always read the accompanying technical datasheet for the specific protein. The below suggestions are general guidelines.Proteins supplied in liquid form
The majority of our proteins are supplied without carrier protein or other additives.
The proteins that are supplied in solution are not needed to be
reconstituted, since the proteins are supplied in liquid state with
adequate buffer. Before opening perform a quick-spin.
You do not need to add any carrier protein, only if you intend to store the vial for long term you could add the carrier protein (please see below under lyophilized proteins).
At arrival please store short-term (2-7 days) at 4°C and for near future use below -20°C. For long term storage we recommend to store at -70°C with the addition of cryopreservative agent (such as glycerol).
Avoid freeze/thaw cycles.Lyophilized proteins
If lyophilized the small amount of protein can be deposited on the vial. Before opening we recommend to quick-spin in a micro centrifuge for approx 30 seconds to make sure that the protein get down to the bottom of the vial.
It is recommended to reconstitute lyophilized protein in sterile H2O to a concentration not less than 100 µg/ml. This solution can then be further diluted into other aqueous solutions.
We recommend the addition of a carrier protein to the reconstituted product. After the initial reconstitution the protein should be diluted into working concentrations using an aqueous buffer containing 0.1% BSA or HSA (or any albumin), or diluted in culture media containing serum, such as FCS (unless otherwise stated on the datasheet).
At arrrival, and if you have reconstituted lyophilized protein, please store short-term at 4°C and for future use in aliquots below -20°C. For long term storage we recommend to store at -70°C with the addition of a carrier protein and/or cryopreservative agent (such as glycerol).
Avoid freeze/thaw cycles since this may cause denaturation of the protein.