Western Blot Special

 
The technique of western blotting is today a standard tool for the specific detection of proteins in a sample. Using chemiluminescent and fluorescent signals, the detection limit of proteins is in the low nanogram range.
 
In addition to an extensive range of primary antibodies for western blot, Nordic Biosite offers a complete range of secondary antibodies and auxiliary reagents for your western blots.



Secondary Antibodies

 

Nordic Biosite offers a broad range of high quality secondary antibodies, ensuring we serve the needs of our customers by supplying both frequently sourced antibodies and less common antibodies. We have now made it easy for you to access the secondary antibodies from our homepage. The secondary antibodies are available with the usual enzyme conjugates such as alkaline phosphatase and horseradish peroxidase and also with a complete range of fluorescent DyLight conjugates.


We offer secondary antibodies from several different species, which in combination with fluorochromes enables you to do multiplex western with several different primary-secondary antibody pairs. By using multiplexed western blot you can compare different proteins in the same samples without risking your sample integrity by stripping the membrane.
 

During the month of June we offer a 10% discount on all our Secondary Antibodies.

 
View all discounted products
 
Please use promotion code NBSA06.

 
 

Loading control

 
The quantity of some proteins in the cell remain reasonably constant and are useful as reference points when normalizing sample quantities between different lanes. This is a far more precise method of monitoring changes in protein contents than protein quantification of the sample lysates.
 
Proteins suitable for loading controls are for example:

Beta Actin, GAPDH and tubulin alpha.

Signal enhancer

 
If you are using chemiluminescent western blot, an easy way of enhancing your signal is to dilute your antibodies with MaxBlot.
 
This is an efficient antibody dilutant that can enhance the net signal up to ten-fold compared to dilution in PBS or TBS.