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Alias names |
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14.3.3, YWHAB; GW128; HS1; KCIP-1
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Specificity |
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This antibody recognizes ~28 kDa of human 14.3.3 beta. It does not react to the other isoforms.
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Protocol |
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Western Blot: 1-2 μg/ml
ELISA: 0.01-0.1 μg/ml
Immunoprecipitation: 2-5 μg/ml
IHC (P): 1:100
Flow cytometry: Not tested
POSITIVE CONTROL: 3T3, Brain tissue
CELLULAR LOCATION: Cytoplasmic
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Substrate/Buffer |
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This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer
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Storage |
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The antibodies are stable for 12 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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References |
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Zhi-jun Luo, et al. Identification of the 14.3.3 Domains Important for Self-association and Raf Binding. J. Bio. Chem 270
(40), 23681–23687, 1995
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Notes |
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The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14.3.3 beta is highly expressed in brain tissue. It is critical to cell transformation and mitotic signaling.
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