14-3-3 epsilon - Detailed product view

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Article Nr PAT-80002-2
Description 14-3-3 epsilon , 1-255aa, Human, Recombinant, E.coli
Sizes 0.5mg
Source/Host E.coli
Species Reactivity human
MW 29 kDa
Alias names   YWHAE, MDS, MDCR, KCIP-1, FLJ45465, 14.3.3 e, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Purity   >95% by SDS PAGE
Sequence   MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW
RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF
YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF
YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE
EQNKEALQDV EDENQ
Concentration   1 mg/ml
Substrate/Buffer   In 20mM Tris pH7.5
Storage   Store at -20 °C. Avoid freeze/thaw cycles
References   Oriente F, et al.(2005) J Biol Chem. ;280(49):40642-9.
Conklin D, et al.(2005) PNAS , 92(17):7892-7896
Notes   The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human 14-3-3 ε was expressed in E.coli and purified by using conventional chromatography techniques.

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