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Alias names |
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YWHA1, YWHAH, 14.3.3, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
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Sequence |
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MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DDMASAMKAV
TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE
KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS
EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT
LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN
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Substrate/Buffer |
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Liquid in 20mM Tris pH 8.0. Molecular Weight: 30.3 kDa (266 aa)
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Storage |
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Store at -20 °C. Avoid freeze/thaw cycles
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References |
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Shigeto Sato., et al. (2006), EMBO. 25, 211–221
Chen J., et al. (2007), J Neurosci Res. 85(8):1724-33
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Notes |
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The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. The 14-3-3η (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3η interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3η, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
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