14-3-3 zeta - Detailed product view

Article Nr	PAT-80006-1
Description	14-3-3 zeta, 1-245aa, Human, His-tagged, Recombinant, E.coli
Sizes	100ug
Source/Host	E.coli
Species Reactivity	human
MW	32 kDa

Accession No		NP_ 663723
Alias names		YWHAZ, KCIP-1, MGC111427, 14.3.3, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Purity		>95% by SDS PAGE
Sequence		MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN
Concentration		1 mg/ml
Substrate/Buffer		Liquid in Phosphate Buffered Saline pH7.4
Storage		Store at -20 °C. Avoid freeze/thaw cycles
References		L Gannon-Murakami., et al. (2002). J. Biol. Chem., 277(26): 23116-23122 Li FQ., et al.(2008). J Cell Biol. 181(7):1141-54.
Notes		The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Supplier		BioSite