BHMT - Detailed product view

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Article Nr PAT-80028-1
Description BHMT (Betaine-homocysteine methyltransferase) Human, His- tag, Recombinant E.coli
Sizes 100ug
Source/Host E.coli
Species Reactivity human
MW 49.2 kDa
Alias names   Betaine-homocysteine methyltransferase
Purity   >95% by SDS-PAGE
Sequence   MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP VGGKKAKKGI LERLNAGEIV
IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL
ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ
QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV
RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFID
LPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPP
ASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM
QQKEATTEQQ LKELFEKQKF KSQ
Concentration   1 mg/ml
Substrate/Buffer   Liquid. 20mM Tris pH7.5, 20% glycerol.
Storage   Store at -20 °C. Avoid freeze/thaw cycles
References   Nandita, et al., Protein Expr Purif (2002) 25:73-80
Breksa, A P et al., Biochemistry (1999) 38:13991-8
Notes   Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT displays differential expression in a model of liver cirrhosis. Recombinant human BHMT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Supplier   BioSite
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