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Alias names |
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Macrophage migration inhibitory factor
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Substrate/Buffer |
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In phosphate-buffered saline (PBS), pH 7.4
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Storage |
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Store at -20 °C. Avoid freeze/thaw cycles
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References |
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Weiser WY., et al (1989) Proc Natl Acad Sci. 86: 7522-7526.
Bernhagen J., et al (1994) Biochemistry. 33: 14144-14155.
Richard B., (1996) FASEB J. 10: 1607-1613.
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Notes |
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The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
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