|
Alias names |
|
Acyl-CoA thioesterase 11, adipose associated isoform BFIT2, ACOT 11, BFIT
|
|
Protocol |
|
The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2, 000 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1, 000 and Immunohistochemistry is 1:100.
|
|
Substrate/Buffer |
|
Liquid in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide
|
|
Storage |
|
Can be stored at 4℃ for up to one month, but store at -20℃ for long term storage. Avoid repeated freezing and thawing cycles
|
|
References |
|
S. H. Adams, et al: (2001) Biochem. J. 360, 135-142
|
|
Contents/Specifications |
|
Anti-human ACOT11 mAb, clone J4B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACOT11 protein
The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography
|
|
Notes |
|
ACOT11 (Acyl-CoA thioesterase11), also known as BFIT, is a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of this protein in mouse has been associated with obesity and alternative splicing results in two transcript variants encoding different isoforms.
|