BIGH3 - Detailed product view

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Article Nr PAT-80236-2
Description Recombinant human BIGH3, 502-683 aa
Sizes 0.5mg
Format liquid
Source/Host E.Coli
Species Reactivity human
MW 19.9 kDa (182 aa)
Accession No   NP_000349
Alias names   Transforming growth factor-beta-induced protein ig-h3, Beta ig-h3, TGFBI, Kerato-epithelin, RGD-CAP
Purity   >95% by SDS PAGE
Protocol   Billings PC., et al. (2002) J Biol Chem. 277, 28003–9.
Munier FL., et al. (2002) Invest Ophthalmol Vis Sci. 43, 949–54.
Kim JE., et al. (2000) J Biol Chem. 275, 30907–15
Sequence   MGTVMDVLKG DNRFSMLVAA IQSAGLTETL NREGVYTVFA PTNEAFRALP PRERSRLLGD AKELANILKY HIGDEILVSG GIGALVRLKS LQGDKLEVSL KNNVVSVNKE PVAEPDIMAT NGVVHVITNV LQPPANRPQE RGDELADSAL EIFKQASAFS RASQRSVRLA PVYQKLLERM KH
Concentration   0, 5 mg/ml
Substrate/Buffer   Liquid. In 20 mM Tris-HCl ( pH 8.0) containing 1 mM EDTA 0.1 mM PMSF, 20% Glycerol
Storage   Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles
Contents/Specifications   Recombinant human BIGH3 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Real molecular size on SDS-PAGE will be shift up
Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
Notes   BIGH3, also known as TGFBI and Beta ig-h3, is an extracellular matrix protein induced by transforming growth factor(TGF)-beta 1. BIGH3 protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of BIGH3 were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain).
Supplier   BioSite
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