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Alias names |
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Eukaryotic translation initiation factor 5A isoform B, EIF-5A, EIF5A1, eIF5AI
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Sequence |
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MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK
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Concentration |
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1 mg/ml (determined by Bradford assay)
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Substrate/Buffer |
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Liquid. In 50 mM Tris-HCl buffer pH 7.5 containing 10 % glycerol
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Storage |
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Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
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References |
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Saini P, et al. (2009). Nature. 459(7243):118-21
Gosslau A., et al. (2009). J Cell Physiol. 219(2):485-93
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Contents/Specifications |
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Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques
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Notes |
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Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.
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