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Alias names |
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A26C1A, A26C1B, ACTB, Actin cytoplasmic 1, Actin, beta antibody, Actx, Beta actin, beta cytoskeletal actin, E430023M04Rik, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1
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Protocol |
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Western blot: At 0.25-0.5μg/ml with the appropriate system to detect β-actin in
cells and tissues.
Immunohistochemistry(P): At 0.5-1μg/ml to detect β-actin in formalin fixed and paraffin embedded tissues.
Other applications have not been tested.
Optimal dilutions should be determined by end user.
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Substrate/Buffer |
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Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.
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Storage |
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At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for longer time.
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References |
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1. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Kedes, L.; Shows, T. : Chromosome 7 assignment of the human beta-actin functional gene (ACTB) and the chromosomal dispersion of pseudogenes. (Abstract) Cytogenet. Cell Genet. 40: 712 only, 1985.
2. Procaccio, V.; Salazar, G.; Ono, S.; Styers, M. L.; Gearing, M.; Davila, A.; Jimenez, R.; Juncos, J.; Gutekunst, C.-A.; Meroni, G.; Fontanella, B.; Sontag, E.; Sontag, J. M.; Faundez, V.; Wainer, B. H. : A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78: 947-960, 2006.
3. Ng, S.-Y.; Gunning, P.; Eddy, R.; Ponte, P.; Leavitt, J.; Shows, T.; Kedes, L. : Evolution of the functional human beta-actin gene and its multi pseudogene family: conservation of the noncoding regions and chromosomal dispersion of pseudogenes. Molec. Cell. Biol. 5: 2720-2732, 1985.
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Contents/Specifications |
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Purified by the goat anti-mouse IgG affinity chromatography.
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Notes |
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The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
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