Bcl2 - Detailed product view

Price: N/A - Request  Buy
Article Nr PAT-80282-0
Description Recombinant human Bcl-2, 1-211 aa, His-tagged
Sizes 20 µg
Source/Host E.coli
Species Reactivity human
MW 25.4 kDa (231 aa)
Accession No   NP_000624
Alias names   B-cell lymphoma protein 2 alpha
Purity   >90% by SDS PAGE
Sequence   MGSSHHHHHH SSGLVPRGSH MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D
Concentration   0.5 mg/ml (determined by Bradford assay)
Substrate/Buffer   Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 20% glycerol 2 mM DTT
Storage   Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles
References   Zamzami N., et al. (1998) Oncogene. 16(17):2265-82.
Fernandez-Sarabia MJ., et al. (1993) Nature. 366(6452):274-5.
Contents/Specifications   Recombinant Bcl-2 protein was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Notes   Bcl-2, also known as B-cell lymphoma protein 2 alpha, is an anti-apoptotic protein located primarily in the outer mitochondrial membrane that blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apotosis. Bcl-2 exerts its anti-apoptotic function through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). The Bcl-2 gene has been related with a number of cancers, including melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity
Supplier   BioSite
Price: N/A - Request  Buy