Activin A - Detailed product view

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Article Nr KBB-103
Description Human Activin A ELISA Kit
Sizes 1 x 96 test
Applications ELISA
Species Reactivity human
Alias names   Activin Beta-A chain, Erythroid differentiation protein, EDF, FRP, Activin-A, Inhibin-B, Inihibin-Beta A chain
Sensitivity   < 12pg/ml
Range   62.5-4000 pg/ml
SampleTypes   serum, plasma, tissue lysate, cell culture supernatants
Specificity   No detectable cross-reactivity with any other cytokine
Storage   Store at 4°C for frequent use, at - 20°C for infrequent use. Avoid freeze-thaw cycles. Expiration time four months at 4°C and eight months at -20°C
References   1. Shav-Tal, Y.; Zipori, D. The role of activin a in regulation of hemopoiesis. Stem Cells. 2002;20(6):493-500.
2. Di, Loreto, C.; Reis, FM.; Cataldi, P.; Zuiani, C.; Luisi, S.; Beltrami, CA.; Petraglia, F. Human mammary gland and breast carcinoma contain immunoreactive inhibin/activin subunits: evidence for a secretion into cystic fluid. Eur J Endocrinol.1999 Aug;141(2):190-4.
Contents/Specifications   1. Lyophilized recombinant human Activin A standard: 10ng/tube×2.
2. One 96-well plate precoated with anti- human Activin A antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- human Activin A antibody : 130μl,dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex(ABC): 130μl,dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Notes   Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
Supplier   BioSite
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