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SampleTypes |
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serum, plasma, tissue lysate, cell culture supernatants
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Specificity |
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No detectable cross-reactivity with any other cytokine
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Storage |
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Store at 4°C for frequent use, at - 20°C for infrequent use. Avoid freeze-thaw cycles. Expiration time four months at 4°C and eight months at -20°C
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References |
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1. Domeij, H.; Modeer, T.; Quezada, HC.; Yucel-Lindberg, T. Cell expression of MMP-1 and TIMP-1 in co-cultures of human gingival fibroblasts and monocytes: the involvement of ICAM-1. Biochem. Biophys. Res. Commun. 2005 Dec 30;338(4):1825-33. Epub 2005 Nov 2.
2. Joos, L.; He, J.-Q.; Shepherdson, M. B.; Connett, J. E.; Anthonisen, N. R.; Pare, P. D.; Sandford, A. J. The role of matrix metalloproteinase polymorphisms in the rate of decline in lung function. Hum. Molec. Genet. 11: 569-576, 2002.
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Contents/Specifications |
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1. Lyophilized recombinant human MMP-1standard: 10ng/tube×2.
2. One 96-well plate precoated with anti- human MMP-1 antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- human MMP-1 antibody : 130μl，dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex（ABC）: 130μl，dilution 1：100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml
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Notes |
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Matrix metalloproteinase-1 (MMP-1) is also known as collagenase. Matrix metalloproteinases (MMPs) are zinc-dependent proteases that degrade extrMMP-1llular matrix proteins. The MMPs comprise a family of at least 20 proteolytic enzymes that play an essential role in tissue remodeling. Matrix metalloproteinase-1 (MMP-1) plays an important role in the degradation of collagen in inflammatory diseases.1 MMP1 (interstitial collagenase), MMP9 (gelatinase B) and MMP12 (macrophage elastase) are thought to be important in the development of emphysema.2 Smoking-induced MMP-1 might be important in the skin-ageing effects of tobacco smoking.3 The standard product used in this kit is human MMP-1 with the molecular mass of 51-53KDa. The detected MMP-1 includes zymogen and active enzyme.
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