BMF - Detailed product view

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Article Nr PAT-80310-1
Description Recombinant human BMF, 1-129 aa, T7 tagged
Sizes 100ug
Format purified
Source/Host E.coli
Species Reactivity human
MW 15.6 kDa (144aa)
Accession No   NP_001003943
Alias names   Bcl2 modifying factor isoform 3
Purity   >90% by SDS-PAGE
Sequence   MASMTGGQQM GRGSHMEPSQ CVEELEDDVF QPEDGEPVTQ PGSLLSADLF AQSLLDCPLS RLQLFPLTHC CGPGLRPTSQ EDKATQTLSP ASPSQGVMLP CGVTEEPQRL FYAPAEPKSC VVADPPLPAQ PCFEWRREQE RGRP
Concentration   1 mg/ml (determined by Bradford assay)
Substrate/Buffer   Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing, 1mM DTT, 10% glycerol. Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method)
Storage   Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles
References   Morales AA., et al. (2004). Leukemia. 18(1):41-7
Chen L., et al. (2005). Mol Cell. 17(3):393-403
Contents/Specifications   Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Notes   Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types.
Supplier   BioSite
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