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Alias names |
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Adipocyte Complement-Related Protein of 30kDa, Acrp30, GBP28, AdipoQ, APDN
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SampleTypes |
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plasma, serum, supernatants, urine
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Specificity |
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This assay recognizes both natural and recombinant rat adiponectin. It can detect both globular domain and full-length adiponectin
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Protocol |
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Rat Adiponectin ELISA Kit is designed for detection of rat adiponectin in plasma,
serum, urine and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures adiponectin in 3.5 hours. A polyclonal antibody specific for adiponectin has been pre-coated onto a microplate. Adiponectin in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for
adiponectin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured
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Storage |
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Store unopened kit at 2-8 C up to expiration date
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References |
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(1) Tsao, T.S. et al. (2002) EJP 440:213-221
(2) Adamczak, M. et al. AJH 16:72-75
(3) Matsubara, M. et al. (2003) Eur J Endocrinol. 148(6): 657-62
(4) Kojima, S. et al. (2003) Heart 89(6): 667
(5) Zoccali, C. et al. (2003) Kidney Int Suppl. 84: S98-102
(6) Pannacciulli, N. et al. (2003) J Clin Endocrinol Metab. 88(4): 174
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Contents/Specifications |
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Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against rat adiponectin.
Sealing: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes, which can be cut to fit the format of the individual assay.
Adiponectin Standard: Recombinant rat adiponectin in a buffered protein base (150 ng, lyophilized).
Biotinylated Adiponectin Antibody (100x): A 100-fold concentrated biotinylated
polyclonal antibody against adiponectin (80 µl).
MIX Diluent Concentrate (10x): 30 ml
Wash Buffer Concentrate (20x): 30 ml
Streptavidin-Peroxidase Conjugate: 100-fold concentrate (90 µl)
Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate
tetramethylbenzidine (8 ml).
Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction (12 ml).
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Notes |
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Adiponectin, also known as Adipocyte Complement-Related Protein of 30kDa (ACRP30), is a secreted serum protein expressed exclusively in differentiated adipocytes. Studies indicated that decreased plasma adiponectin concentration is associated with obesity, insulin resistance (1), essential hypertension (2), inflammation and atherosclerosis (3), and acute myocardial infarction (4). On the other hand, increased adiponectin level leads to nephrotic syndrome (5, 6).
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