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Sequence |
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MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK
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Concentration |
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1 mg/ml (determined by Bradford assay)
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Substrate/Buffer |
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Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
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Storage |
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Can be stored at +4°C short term (1-2 week). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles
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References |
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Kravets A., et al. (2004) J Biol Chem. 279(19):19916-23.
Franklin E., et al. (2007) Biochem J. 405(1):61-7.
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Contents/Specifications |
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Real molecular weight on SDS-PAGE will be shift up
Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method )
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Notes |
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BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond.( Bilirubin + NAD(P)+ = biliverdin + NAD(P)H ) Recombinant BLVRA protein was expressed in E.coli and purified by using conventional chromatography techniques
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