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Alias names |
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14.3.3, YWHAS, Stratifin, SFN, HME1
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Sequence |
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MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS
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Concentration |
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1 mg/ml (determined by Bradford assay)
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Substrate/Buffer |
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Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM NaCl 10% glycerol
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Storage |
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Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles
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References |
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Benzinger A., et al. (2005) Mol Cell Proteomics. 4(6): 785-95.
Wilker E W., et al. (2005) J Biol Chem. 280:18891-18898
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Contents/Specifications |
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Recombinant human 14-3-3 sigma was expressed in E.coli and purified by using conventional chromatography techniques
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Notes |
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14-3-3 sigma , also known as Stratifin (SFN), belong to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis.
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