BAG3 - Detailed product view

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Article Nr PAT-80415-1
Description Recombinant human BAG3, 1- 575aa, His-tagged
Sizes 50ug
Format purified
Source/Host E.coli
Species Reactivity human
MW 63.7 kDa (595aa)
Accession No   NP_004272
Alias names   BAG family molecular chaperone regulator 3, BIS, CAIR-1
Purity   >90% by SDS-PAGE
Sequence   MGSSHHHHHH SSGLVPRGSH MSAATHSPMM QVASGNGDRD PLPPGWEIKI DPQTGWPFFV DHNSRTTTWN DPRVPSEGPK ETPSSANGPS REGSRLPPAR EGHPVYPQLR PGYIPIPVLH EGAENRQVHP FHVYPQPGMQ RFRTEAAAAA PQRSQSPLRG MPETTQPDKQ CGQVAAAAAA QPPASHGPER SQSPAASDCS SSSSSASLPS SGRSSLGSHQ LPRGYISIPV IHEQNVTRPA AQPSFHQAQK THYPAQQGEY QTHQPVYHKI QGDDWEPRPL RAASPFRSSV QGASSREGSP ARSSTPLHSP SPIRVHTVVD RPQQPMTHRE TAPVSQPENK PESKPGPVGP ELPPGHIPIQ VIRKEVDSKP VSQKPPPPSE KVEVKVPPAP VPCPPPSPGP SAVPSSPKSV ATEERAAPST APAEATPPKP GEAEAPPKHP GVLKVEAILE KVQGLEQAVD NFEGKKTDKK YLMIEEYLTK ELLALDSVDP EGRADVRQAR RDGVRKVQTI LEKLEQKAID VPGQVQVYEL QPSNLEADQP LQAIMEMGAV AADKGKKNAG NAEDPHTETQ QPEATAAATS NPSSMTDTPG NPAAP
Concentration   0.5 mg/ml (determined by Bradford assay)
Substrate/Buffer   Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM EDTA, 0.1mM PMSF, 10% glycerol
Storage   Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
References   Virador VM., et al. (2009) PLos One. 4(4):e5136.
Kyratsous CA., et al. (2008) Proc Natl Acad Sci U S A. 105(52):20912-7
Contents/Specifications   Recombinant human BAG3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Notes   BAG3 was reported initially as a protein-refolding cochaperone of the bcl2 binding protein BAG family and as upregulated in response to persistent stress of cellular calcium balance dysregulation. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. Also, it has been shown to diminish stress-induced apoptosis
Supplier   BioSite
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