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Alias names |
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EIF4EBP1, 4E-BP1, 4EBP1, PHAS-I
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Specificity |
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4E-BP1 (G39) antibody detects endogenous levels of 4E-BP1 protein
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Protocol |
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WB: 1:500~1:1000 IHC: 1:50~1:200
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Substrate/Buffer |
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1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2
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Storage |
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles
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Notes |
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The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.
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