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Alias names |
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ACTN 1, ACTN1, Actinin alpha 1, Alpha actinin cytoskeletal isoform, F actin cross linking protein, FLJ40884, Non muscle alpha actinin 1
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Specificity |
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Actinin-α3 (E25) antibody detects endogenous levels of Actinin-α3 protein
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Protocol |
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WB: 1:500~1:1000 IF: 1:50~1:200
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Substrate/Buffer |
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1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2
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Storage |
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles
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Notes |
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Focal adhesions were identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix.Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex, which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and talin complex or by a direct interaction with integrin..
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