Actinin alpha 3 (E25) - Detailed product view

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Article Nr BT-BS2205
Description Anti-Actinin-α3 (E25) (affinity purified)
Sizes 100 ug
Format affinity purified
Source/Host rabbit
Clone polyclonal
Applications IF, WB
Species Reactivity human, mouse, rat
Alias names   ACTN 1, ACTN1, Actinin alpha 1, Alpha actinin cytoskeletal isoform, F actin cross linking protein, FLJ40884, Non muscle alpha actinin 1
Specificity   Actinin-α3 (E25) antibody detects endogenous levels of Actinin-α3 protein
Protocol   WB: 1:500~1:1000 IF: 1:50~1:200
Concentration   1 mg/ml
Substrate/Buffer   1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2
Storage   Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles
Notes   Focal adhesions were identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix.Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex, which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. α-actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and talin complex or by a direct interaction with integrin..
Supplier   BioSite
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