Apo CIII - Detailed product view

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Article Nr KSP-124
Description Monkey Apolipoprotein C-III ELISA
Sizes 1x96 test
Format precoated
Applications ELISA
Species Reactivity human, monkey
Alias names   Apo-CIII, ApoC-III, ApoCIII, Apolipoprotein C-III
Sensitivity   0.1 µg/ml
Range   0.078-5.000 µg/ml
SampleTypes   plasma, serum, cell culture
Specificity   No significant cross reactivity with Apo AI, Apo AII, Apo B, Apo CI, Apo CII, or Apo E. This kit has 100% cross reactivity with Monkey samples
Protocol   This assay employs a quantitative competitive enzyme immunoassay technique that measures monkey Apo C-III in less than 5 hours. A mAb capture antibody has been coated onto a 96-well microplate with removable strips. Monoclonal antibody against monkey Apo C-III binds to the captured antibody. Apo C-III in standards and samples is competed by a biotinylated Apo C-III sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Storage   Store unopened kit at 2-80C up to expiration date.
References   (1) Jong MC et al. (1999) Arterioscler. Thromb. Vasc. Biol. 19: 472-484
(2) Wang CS et al. (1985) J. Clin. Invest 75: 384-390
(3) Dammerman M et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90: 4562–4566
(4) Ito Y et al. (1990) Science 249: 790-793
(5) Aalto-Setala K et al. (1996) J. Lipid Res. 37:1802-1811
(6) Gerritsen G et al. (2005) J. Lipid Res. 46:1466-1473
(7) Duivenvoorden I et al. (2005) Diabetes 54:664-671
Contents/Specifications   • 96-well polystyrene mAb capture microplate (12 strips of 8 wells)
• Monoclonal Antibody against Monkey Apo C-III (40x): 1 vial, (180μl)
• Sealing Tapes: 3 pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
• Monkey Apo C-III Standard: buffered protein base (5 μg, lyophilized).
• Biotinylated Apo C-III (1x): lyophilized, 1 vial
• EIA Diluent Concentrate (10x) (30 ml).
• Wash Buffer Concentrate (20x) (30 ml, 2 bottles).
• Streptavidin-Peroxidase Conjugate (SP Conjugate) 100-fold concentrate (80 μl)
• Chromogen Substrate: ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine (8 ml).
• Stop Solution: 0.5 N hydrochloric acid to stop the chromogen substrate reaction (12 ml)
Notes   Apolipoprotein C-III (apoC-III) is a surface component of chylomicrons, very low density lipoproteins, and high density lipoproteins. It consists of 79 amino acids with a molecular mass of 8.8 kDa (1). ApoC-III is synthesized mainly in the liver and to a lesser degree in the intestine. It plays a key role in triglyceride-rich lipoprotein metabolism. It is an inhibitor of lipoprotein lipase and hepatic lipase, and interferes with binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors (2 -3). Overexpression of the human apoC-III gene causes hypertriglyceridemia in transgenic mice (4 - 5). Deficiency of apoC-III prevents hyperlipidemia induced by apoE overexpression (6). As its deficiency results in diet-induced obesity and aggravated insulin resistance in mice, apoC3 is a potential target for treatment of obesity and insulin resistance (7).
Supplier   BioSite
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