|
Alias names |
|
ARG, ABLL, FLJ22224, FLJ31718, FLJ41441, ABL2, Abelson-related gene, Abl-related gene
|
|
Protocol |
|
Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|
|
Substrate/Buffer |
|
Ascitic fluid containing 0.03% sodium azide.
|
|
Storage |
|
Store at 4℃, for long term storage, store at -20℃.
|
|
References |
|
1. Yoshimi I, Takashi I, Tsuneyuki O, et al. Blood. 2000; 95(6): 2126-2131.
2. Scheijen, B. and Griffin, J.D. Oncogene. 2002); 21：3314-33.
|
|
Notes |
|
ABL2(ARG, Abelson-related gene) is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells. The ABL2 gene product is expressed as two variants bearing different amino termini, both approximately 12-kb in length. c-Abl shows both cytoplasmic and nuclear localization，c-Abl is involved in two different chromosomal translocations present in human leukemias, which generate Bcr-Abl and TEL-Abl.
|