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Protocol |
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Add 500 ul deionized water to the vial to prepare a working stock solution at 200 ug/mL . Allow to set at least 30 minutes at 4 ° C, mix well.
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Sequence |
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153 SIPWNLERIT PPRYRADEYQ PPDGGSLVEV YLLDTSIQSD HREIEGRVMV TDFENVPEED GTRFHRQASK CDSHGTHLAG VVSGRDAGVA KGASMRSLRV LNCQGKGTVS GTLIGLEFIR KSQLVQPVGP LVVLLPLAGG YSRVLNAACQ RLARAGVVLV TAAGNFRDDA CLYSPASAPE VITVGATNAQ DQPVTLGTLG TNFGRCVDLF APGEDIIGAS SDCSTCFVSQ SGTSQAAAHV AGIAAMMLSA EPELTLAELR QRLIHFSAKD VINEAWFPED QRVLTPNLVA ALPPSTHGAG WQLFCRTVWS AHSGPTRMAT AVARCAPDEE LLSCSSFSRS GKRRGERMEA QGGKLVCRAH NAFGGEGVYA IARCCLLPQA NCSVHTAPPA EASMGTRVHC HQQGHVLTGC SSHWEVEDLG THKPPVLRPR GQPNQCVGHR EASIHASCCH APGLECKVKE HGIPAPQEQV TVACEEGWTL TGCSALPGTS HVLGAYAVDN TCVVRSRDVS TTGSTSEGAV TAVAICCRSR HLAQASQELQ 692
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Substrate/Buffer |
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Lyophilized 100 ug human PCSK9 in 100 ul of TBS (20 mM Tris, 50mM NaCl, pH8.0 ), Carry free.
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Storage |
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Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
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Contents/Specifications |
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A DNA sequence encoding the human PCSK9 (Ser153-Gln692) with 6 His tag on the N-Terminus was expressed in E. Coli. This protein was purified by Ni-NTA column.
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