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Protocol |
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Add 500 ul deionized water to the vial to prepare a working stock solution at 200 ug/mL . Allow to set at least 30 minutes at 4 ° C, mix well.
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Sequence |
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109YM YHSAFSVGLE TRVTVPNVPI RFTKIFYNQQ NHYDGSTGKF HCNIPGLYYF SYHITVYMKD VKVSLFKKDK AVLFTYDQYQ EKNVDQASGS MLLHLEVGDQ VWLQVYGEGD NNGLYADNVN DSTFTGFLLY HDTN 244
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Substrate/Buffer |
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Lyophilized 100 ug Rat Adiponectin, globular form in 50 ul of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ), Carry free.
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Storage |
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Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
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Contents/Specifications |
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A DNA sequence encoding the Rat Adiponectin globular form (Ala108-Asn244) with 6 His tag on the N-Terminus was expressed in E. Coli . This protein was purified by Ni-NTA column.
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