|
Protocol |
|
Add 500 ul deionized water to the vial to prepare a working stock solution at 200 ug/mL . Allow to set at least 30 minutes at 4 ° C, mix well.
|
|
Sequence |
|
1
MLLLGAVLLL LALPGHDQET TTQGPGVLLP LPKGACTGWM
AGIPGHPGHN GAPGRDGRDG TPGEKGEKGD PGLIGPKGDI
GETGVPGAEG PRGFPGIQGR KGEPGEGAYV YRSAFSVGLE
TYVTIPNMPI RFTKIFYNQQ NHYDGSTGKF HCNIPGLYYF
AYHITVYMKD VKVSLFKKDK AMLFTYDQYQ ENNVDQASGS
VLLHLEVGDQ VWLQVYGEGE RNGLYADNDN DSTFTGFLLY
HDTN 244
|
|
Substrate/Buffer |
|
Lyophilized 100 ug human Adiponectin in 50 ul of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ), Carry free.
|
|
Storage |
|
Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
|
|
Contents/Specifications |
|
A DNA sequence encoding the human Adiponectin full length (Met1-Asn244) with 6 His tag on the N-Terminus was expressed in E. Coli . This protein was purified by Ni-NTA column.
|