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Protocol |
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Add 500 μl deionized water to the vial to prepare a working
stock solution at 200 μg/mL . Allow to set at least 30 minutes at
4 ° C, mix well.
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Sequence |
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293 SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW
QAFYCHGDCP FPLADHLNST NHAIVQTLVN
CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR 408
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Substrate/Buffer |
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Lyophilized 100 μg human bone morphogenetic protein 4
μl of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ). Carry free.
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Storage |
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Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein
is stable for up to 6 months from date of receipt at -20 °C to - 70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long
term storage (six months). Aliquot reconstituted protein to avoid
repeated freezing / thawing cycles.
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Contents/Specifications |
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A DNA sequence encoding the mature human bone
morphogenetic protein 4 (Ser293-Arg408) was recombined with
Hexa-histidine tag at the N-Terminus. That was expressed in
E.Coli, and purified by Ni-NTA column
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