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Protocol |
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Add 500 ul deionized water to the vial to prepare a working stock solution at 200 ug/mL . Allow to set at least 30 minutes at 4 ° C, mix well.
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Sequence |
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32PELSLKGTQ HIMQAGQTLH LQCRGEAAHK WSLPEMVSKE
SERLSITKSA CGRNGKQFCS TLTLNTAQAN HTGFYSCKYL
AVPTSKKKET ESAIYIFISD TGRPFVEMYS EIPEIIHMTE
GRELVIPCRV TSPNITVTLK KFPLDTLIPD GKRIIWDSRK
GFIISNATYK EIGLLTCEAT VNGHLYKTNY LTHRQTNTII
DVQISTPRPV KLLRGHTLVL NCTATTPLNT RVQMTWSYPD
EKNKRASVRR RIDQSNSHAN IFYSVLTIDK MQNKDKGLYT
CRVRSGPSFK SVNTSVH 327
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Substrate/Buffer |
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Lyophilized 100 ug human VEGF-R1/Flt-1 (32-327) in 100 ul of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
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Storage |
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Store lyophilized protein at -20 °C or -70 °C. Lyophilized protein is stable for up to 6 months from date of receipt at - 20 °C to -70 °C. Upon reconstitution, this protein can be stored at -20 °C for a few weeks or at -70 °C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
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Contents/Specifications |
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A DNA sequence encoding the N-terminal extracellular domain of human VEGF-R1/Flt-1(Phe32-His327) with 6 His tag on the N-Terminus was expressed in E. Coli. This protein was purified by Ni-NTA column.
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