|
Specificity |
|
This antibody has been selected for its ability to recognize human sRAGE in direct ELISAs and western blots as well as immunohistochemistry.
|
|
Protocol |
|
Add 100 ul of PBS to the vial to prepare antibody stock solution at 100 ug/100 ul.
Direct ELISA - This antibody can be used at 1:4000 (0.25 ug/ml) with the appropriate secondary reagents to detect recombinant human sRAGE.
Western blot - This antibody can be used at 1: 500~ 1000 [1~2 μg/mL ] with the appropriate secondary reagents to detect rh sRAGE or human serum samples at 38KDa under reduce condition . The detection limit for rh sRAGE approximately 10 ng/lane under reduce conditions.
Immunohistochemistry-That Antibody can be used at 1: 500 with the appropriate secondary antibody to detect sRAGE in human kidney tissues (ABC).
|
|
Substrate/Buffer |
|
100 μg of rabbit anti-human sRAGE Purified IgG in 100 ul of PBS without preservatives was lyophilized.
|
|
Storage |
|
Store reconstituted antibody at 2 to 8 ° C for up a few weeks. This antibody can also be aliquotted ( by 10 uL per vial) and stored frozen at -20° C to -70° C in a manual defrost freezer for up six months without detectable loss of activity.
|
|
Contents/Specifications |
|
This antibody was produced from a rabbit immunized with purified, E. coli-derived, recombinant human soluble RAGE. That was purified with Protein A affinity.
|