ANGPTL4 - Detailed product view

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Article Nr ABE-309-01
Description Human ANGPTL-4 ELISA Kit
Sizes 96T
Applications ELISA
Species Reactivity human
Sensitivity   0.15 ng/mL
Range   Standard: 0.3125-40 ng/mL
SampleTypes   Serum, EDTA Plasma, cell culture
SampleSize   100 ul
Specificity   This assay recognizes both natural and recombinant human ANGPTL4. The factors listed below were prepared at 800 ng/mL in Dilution Buffer, and assayed for cross reactivity. Preparations of the following factors at 50 ng/mL in a mid-range rh ANGPTL4 control were assayed for interference. No significant cross-reactivity or interference was observed.
Storage   2-8 °C
Contents/Specifications   This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ANGPTL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGPTL4 present is bound by the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific for ANGPTL4 is added to the wells. Following a wash to remove any unbound
antibody-biotin reagent, HRP link Streptavidin is add to the wells. After washing away any unbound enzyme, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPTL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Intra-assay Precision 4-6%, Inter-assay Precision 8-10%
Notes   Human ANGPTL4 immunoassay is a 3.5 - 4.5 hour solid phase ELISA designed to measure human ANGPTL4 in cell culture supernates, serum, and plasma. It contains recombinant human ANGPTL4 and antibodies raised against this protein. It has been shown to accurately quantitie recombinant human ANGPTL4. Results obtained with naturally occurring ANGPTL4 samples showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that the Immunoassay kit can be used to determine relative mass values for natural human ANGPTL4.
Supplier   BioSite
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