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SampleTypes |
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Serum, EDTA plasma, cell culture
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Specificity |
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Mouse Vaspin ELISA kit recognizes recombinant and endogenous mouse Vaspin. The data also indicated that rat serum samples were competitively bound to antibody that was used in this kit formulation condition. Its linear dilution curves were parallel to the standard curves obtained using the ELISA standard. That means rat serum samples cross-react with mouse Vaspin ELISA kit.
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References |
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1: Caminos JE, et al. Vaspin and amylin are expressed in human and rat placenta and regulated by nutritional status. Histol Histopathol. 2009 Aug; 24(8):979-90.
2: González CR, et al. Regulation of visceral adipose tissue-derived serine protease inhibitor by nutritional status, metformin, gender and pituitary factors in rat white adipose tissue. J Physiol. 2009 Jul 15;587(Pt 14):3741-50. Epub 2009 May
26.
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Contents/Specifications |
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Intra-assay Precision 6-8%
Inter-assay Precision 8-12%
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Notes |
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Mouse Vaspin ELISA employs the quantitatively competitive enzyme immunoassay technique in which Mouse Vaspin present in samples competed with a fixed amount of biotinylated Mouse Vaspin for sites on purified rabbit IgG specific against Mouse Vaspin. During the incubation, the rabbit IgG becomes bound to the goat anti-rabbit IgG pre-coated onto the microplates. Following a wash to remove any unbound antibody, standard, samples and biotin conjugate, a Streptavidin conjugated to horseradish-peroxidase (HRP) is added to the wells. After washing away any unbound enzyme, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of Mouse Vaspin bound in the initial step. The sample values are then read off the standard curve.
Mouse Vaspin ELSA has been shown to accurately quantitate the recombinant and natural Mouse Vaspin. Results obtained using natural Mouse Vaspin showed dose response curves that were parallel to the standard curves obtained using the kit standards.
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