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Alias names |
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SNA; SNAH; SLUGH2; dJ710H13.1; SNAI1, Snail
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Protocol |
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Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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Substrate/Buffer |
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Ascitic fluid containing 0.03% sodium azide
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Storage |
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Store at 4℃, for long term storage, store at -20℃
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References |
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1. Exp Cell Res. 2008 Aug 1;314(13):2448-53.
2. Mol Cell Biol. 2008 Aug;28(15):4772-81
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Notes |
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Snail is a zinc-finger transcription factor that can repress E-cadherin transcription. Downregulation of E-cadherin is associated with epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells . Indeed, loss of E-cadherin expression is correlated with the invasive properties of some tumors and there is a considerable inverse correlation between Snail and E-cadherin mRNA levels in epithelial tumor cell lines . In addition, Snail blocks the cell cycle and confers resistance to cell death . Phosphorylation of Snail by GSK-3 and PAK1 regulates its stability, cellular localization and function .Tissue specificity: Expressed in a variety of tissues with the highest expression in kidney.
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