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Activity |
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Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
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Protocol |
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Claesson-Welsh L. (2008) Arterioscler Thromb Vasc Biol. 28(9):1575-6.
Malik AK, , et al. (2006) Blood. 107(2):550-7.
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Concentration |
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1 mg/ml (determined by Bradford assay)
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Substrate/Buffer |
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In 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
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Storage |
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Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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References |
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1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast )
2. Maintenance Condition: RPMI 1640 containing 10% FBS
3. Assay medium: serum free RPMI 1640
4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul)
5. Incubation time : 24 hr (after sample treatment)
6. Concentration range: 10pg/ml - 10ug/ml
7. Detection method : BrdU assay
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Contents/Specifications |
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Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
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Notes |
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VEGF-B, also known as Vascular endothelial growth factor-B, is one of 5 mammalian VEGF family members, the others being VEGF-A, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-B binds to one of the VEGF receptor tyrosine kinases, namely VEGF receptor-1(VEGFR1, Flt1), but not to VEGFR2 and VEGFR3. VEGF-B is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis. Recombinant VEGF-B protein was expressed in E.coli and purified by using conventional chromatography techniques.
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