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Alias names |
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Chicken anti-rabbit IgG, Rabbit IgG, DyLight
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Purity |
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Affinity purified antibody is > 95% based on SDS-PAGE
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Protocol |
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The optimal working dilution should be determined by the investigator. Suggested starting dilution(s):
1:20 – 1:2, 000 for most applications
Rehydrate with 1.1 ml of deionized water and let stand at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
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Concentration |
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1 mg/ml (E 1% at 280 nm = 13.2)
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Substrate/Buffer |
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10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.05% (w/v) Sodium Azide
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Storage |
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Store freeze-dried powder at 2-8⁰C.
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Contents/Specifications |
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Purification: Affinity purified using solid phase rabbit IgG (H&L)
DyLight® 800 (Ex = 777 nm; Em = 794 nm)
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