IgG (H&L) - Detailed product view

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Article Nr IR-GtBo-003D3
Description Goat anti-Bovine IgG (H&L) - Affinity Pure, DyLight®350 Conjugate
Sizes 1.0 mg
Format DyLight 350
Source/Host goat
Immunogen Purified Bovine IgG, whole molecule
Applications FC, IMC
Species Reactivity cow/bovine
Alias names   Goat anti-bovine IgG, Bovine IgG
Purity   Affinity purified antibody is > 95% based on SDS-PAGE
Protocol   The optimal working dilution should be determined by the investigator. Suggested starting dilution(s):
1:20 – 1:2, 000 for most applications


Rehydrate with 1.1 ml of deionized water and let stand at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Concentration   1 mg/ml (E 1% at 280 nm = 13.0)
Substrate/Buffer   10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.05% (w/v) Sodium Azide
Storage   Store freeze-dried powder at 2-8⁰C.
Contents/Specifications   Purification: Affinity purified using solid phase bovine IgG (H&L)

DyLight® 350 (Ex = 353 nm; Em = 432 nm)
Supplier   BioSite
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