|
Storage |
|
Store components of the kit at 2-80C or -200C upon arrival up to the expiration date.
|
|
References |
|
(1) Gekle M. (2004) Annu Rev Physiol.
(2) Schindler C et al. (1999) J Hepatol. 31(6): 1132
(3) Hemmelder MH et al. (1997) Nephrol Dial Transplant. 12 Suppl 2:57-62
(4) Sesmilo G et al. (2004) Ann Intern Med. 133(2): 111-22
(5) Wettstein R et al. (2004) Shock. 22(4): 351-357
(6) Saito T et al. (1991) Jpn J Surg. 21(4): 402-11
(7) Strand TA (2004) Am J Clin Nutr. 79(3): 451-6
|
|
Contents/Specifications |
|
• Rat Albumin Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against rat albumin.
• Sealing Tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
• Rat Albumin Standard: Rat albumin in a buffered protein base (150 μg, lyophilized).
• Biotinylated Albumin: 1 vial, lyophilized.
• MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base (30 ml).
• Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant (30 ml).
• Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate (80 μl).
• Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine (8 ml).
• Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction (12 ml).
|
|
Notes |
|
Albumin is serum hepatic protein, the most abundant protein in serum and contributes to the maintenance of oncotic pressure as well as to transport of hydrophobic molecules (1). Serum albumin level has been linked in clinical practice to several diseases. Low albumin levels can suggest liver (2), kidneys disease (3), inflammation (4), shock (5), and malnutrition (6). On the other hand, high albumin levels usually reflect dehydration (7).
The Rat Albumin ELISA (Enzyme-Linked Immunosorbent Assay) kit employs a quantitative competitive enzyme immunoassay technique that measures rat plasma and serum albumin in less than 2 hours. A polyclonal antibody specific for rat albumin has been pre-coated onto a 96-well microplate with removable strips. Albumin in standards and samples is competed with a biotinylated Albumin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
|