DNaseI - Detailed product view

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Article Nr AIC-1008
Description Mouse monoclonal antibody to bovine DNaseI
Sizes 100 µg
Source/Host mouse
Immunogen Recombinant full length bovine DNaseI produced usingCHO-based suspension cell line. Antigenis purified from cell culture supernatant
Clone 4B10
Isotype IgG1
Applications ELISA, WB
Species Reactivity cow/bovine
Accession No   P00639
Alias names   DNL1
Specificity   Bovine, cross reactivity to Human
Purity   Protein G affinity chromatography
Protocol   Western immunoblotting, recommended dilution fordetection of bovine DNase I 1:5 000 to 1:10 000, for human DNaseI 1:1000.
Antibody working titer has to be established practically for each particular antigen and assay format
Concentration   1 mg/ml
Substrate/Buffer   PBS pH 7.4
Storage   Store at - 20 °C-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Notes   Bovine deoxyribonuclease I(DNase I) degrades single- and double-stranded DNA. The enzyme works bycleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments
Supplier   BioSite
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