HCV subtype 1b NS5B protein - Detailed product view

Article Nr	AIC-1036
Description	Mouse monoclonal antibody to HCV subtype 1b NS5B protein
Sizes	100 µg
Format	purified
Source/Host	mouse
Immunogen	RecombinantHepatitis C Virus subtype 1b nonstructural protein 5B (NS5B) RNA-dependent RNApolymerase (RdRp)
Clone	10D7
Isotype	IgG1
Applications	ELISA, WB
Species Reactivity	human

Accession No		Q9WMX2
Specificity		Human, HCV subtype 1b NS5B, Epitope mapped to aminoacids 92-105 (TPPHSARSKFGYGA)
Purity		purified by protein G affinity chromatography
Protocol		ELISA titer; 1:1000, Western immunoblotting, reduced conditions; dilution 1:1000. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Concentration		1 mg/ml
Substrate/Buffer		PBS pH 7.4, with 0.1% sodium azide
Storage		Storeat - 20 °C-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiplefreeze-thaw cycles as product degradation may result.
References		Andrei Nikonov, Erkki Juronen and Mart Ustav (2008).  Functional characterization of fingerssubdomain-specific monoclonal antibodies inhibiting the hepatitis C virusRNA-dependent RNA polymerase. J. Biol Chem. 283(35):24089-102.
Notes		Non-structural protein 5B (NS5B)represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, whichis a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronichepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunitRNA-dependent RNA polymerase is absolutely essential for the viral replication.
Supplier		BioSite