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Specificity |
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Human, HCV subtype 1b NS5B, Epitope mapped to aminoacids 111-130 (LSSKAVNHIRSVWKDLLEDT)
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Purity |
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purified by protein G affinity chromatography
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Protocol |
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ELISA titer: 1:1000. Western immunoblotting, reduced conditions; dilution 1:1000. Immunofluorescense: 1:1000.
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
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Substrate/Buffer |
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PBS pH 7.4, with 0.1% sodium azide
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Storage |
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Storeat - 20 °C-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result.
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Notes |
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Non-structural protein 5B (NS5B)represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, whichis a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronichepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunitRNA-dependent RNA polymerase is absolutely essential for the viral replication.Antibodyis derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mousehybridoma culture. cDNA of antibody variable regions were isolated and clonedinto the human IgG1 constant region-containing antibody expression vector.Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cellline. Antibody is used for high-specific efficient detection of soluble ormembrane-bound proteins fused with 9A2 epitope-tag sequence. For moreinformation please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview
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