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Purity |
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>95%. Recombinant protein migrates as ~65 kDa band (reducing conditions) due to glycosylation.
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Activity |
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Human Ang25-R preparation contains less than 0.2 ng/ml endotoxin/ug of protein. It is biological active as tested by the ability to bind immobilized Tie-2/Fc in functional ELISA.
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Protocol |
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The control immunogenic peptide sequences is 85% conserved in pig, 71% in human, and 55% in chicken Ang-2. Ang-2 control peptide has no significant sequence homology with Ang-1, Ang-3, ang-4 or ARPs. The immunogenic peptide sequences is 71% homologous with human Ang-2. The control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity.
Reconstitute in the original vol. of water (dissolve the entire contents in water by mixing or light vortexing at room temp).
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Substrate/Buffer |
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solution or lyophilized, 100mM Tris, pH7.5
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Storage |
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Short-term: unopened, undiluted vials for less than a week at 4 C. Long-term: at -20 C or below in suitable aliquots after reconstitution. Do not freeze and thaw. Stock solutions should be realiquoted and stored frozen at -20 C or below. Do no store working or highly diluted peptides. Stability: 6-12 months at -20C or below.
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Notes |
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Angiopoietin-1 is an angiogenic secreted protein that interact with endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 may play a critical role in heart development. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496 AA: ~85% identity) has recently been identified. It may act an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology. It is prominently expressed in tissues that are in involved in vascular remodeling (ovary, placenta, and uterus).
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