BAFF - Detailed product view

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Article Nr PPT-53313
Description Recombinant Human BAFF (BLyS) (soluble B Lymphocyte Stimulator (BAFF/BLyS))
Sizes 5 µg
Format lyophilized
Source/Host E. Coli
MW 17007 Dalton
Purity   >95.0% as determined by: Analysis by RP-HPLC, Anion-exchange FPLC, SDS-PAGE Silver Stained gel
Activity   Recombinant B Lymphocyte Stimulator is fully biologically active when compared to standard. The activity is determined by the dose-dependant stimulation of IL-8 production by human PBMC. The ED50 for this effect is less than 10ng/ml.
Please note: results may vary with PBMC donors.
Protocol   It is recommended to reconstitute the lyophilized BAFF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280.
2. Analysis by RP-HPLC, using a standard solution of BAFF as a Reference Standard.
Sequence   The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Val-Gln-Gly.

Recombinant Human B Lymphocyte Stimulator produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17007 Dalton
Storage   Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BAFF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles
Notes   The TNF-related ligand, B cell-activating factor belonging to the TNF family (BAFF), is necessary for normal B cell development and survival, and specifically binds the receptors transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI), B cell maturation Ag (BCMA), and BAFF-R.Using specific mAbs, BAFF-R was found to be the predominant BAFF receptor expressed on peripheral B cells, in both humans and mice, and antagonist mAbs to BAFF-R blocked BAFF-mediated costimulation of anti- micro responses. The other BAFF receptors showed a much more restricted expression pattern, suggestive of specialized roles

Gene:
Name:TNFSF13B
Synonyms:BAFF, BLYS, TALL1, ZTNF4
ORFNames:UNQ401/PRO738
Supplier   BioSite
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