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Specificity |
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The E.coli derived recombinant protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2-119. Immunoreactive with sera of HCV-infected individuals
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Purity |
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>95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. Purified by proprietary chromatographic technique.
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Protocol |
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Recombinant HCV Core Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problemsHepatitis C Virus core proteins are purified by proprietary chromatographic techniques
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Substrate/Buffer |
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50mM Tris-HCl, pH-8, 60mM NaCl, 10mM glutathione, 0.25% sarkosil and 50% glycerol
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Storage |
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Recombinant HCV Core is shipped at ambient temperature. Upon arrival, Store at -20C. Recombinant HCV Core is stable 5 years frozen, One month in solution at room temperature
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References |
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1. Effect of hepatitis C virus core protein on modulation of cellular proliferation and apoptosis in hilar cholangiocarcinoma. Chen RF, Li ZH, Chen JS, Hepatobiliary Pancreat Dis Int 2005 Feb;4(1):71-4
2. Hepatitis C Virus Core Protein Modulates TRAIL-Mediated Apoptosis by Enhancing Bid Cleavage and Activation of Mitochondria Apoptosis Signaling Pathway. Chou AH, Tsai HF, Hu CY, J Immunol 2005 Feb 15;174(4):2160-6
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Notes |
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Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways In addition, HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
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