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Protocol |
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Western blot, ELISA, IP, and IHC in lipid metabolic research
Enzyme Immunoassay (ELISA), 3-6ug/ml
Western Blotting 1:1, 000 – 1:2, 000
Immunoprecipitation, IHC staining, working concentration depends on end user’s protocol
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Substrate/Buffer |
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PBS, pH 7.4 with 0.05% Sodium Azide
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Storage |
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Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stale at 4°C for 3 months.
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Notes |
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Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
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