Pepsin Solution (Ready-To-Use), IVD
Pepsin Solution is a ready-to-use solution developed for enzymatic epitope retrieval on formalin-fixed tissue sections onslides. This procedure (sometimes called PIER, Protease Induced Epitope Retrieval) is primarily used in immunohistochemical staining procedures.
Pepsin solution is intended for in vitro diagnostic use.
Pepsin Solution is suitable for enzymatic epitope retrieval carried out after the dewaxing and rehydration of the tissue sections.
1. Cover deparaffinised and rehydrated tissue sections with ready-to-use Pepsin Solution.
2. Incubate for 10 - 15 minutes at 37&<76;C or 20 &S211; 30 minutes at room temperature. The optimal incubation time needs to be elaborated by the operator. It was shown that in individual cases a stronger signal can be obtained when the incubation time is elongated up to 120 minutes (e. g. for detection of Collagen IV with different antibodies).
3. Rinse carefully (3 x) with wash buffer.
4. Proceed with immunohistological staining as usual.
Varma M, Linden MD, Amin MB Mod Pathol 12:472-478, 1999
Nadji M and Morales AR Ann N.Y. Acad Sci 420:134-9, 1983
2 - 8°C
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