Science Hub

Immunology

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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ELISA 101 Part 2: ELISA Standards

ELISA (Enzyme Linked Immuno-Sorbent Assay) is a highly sensitive plate-based assay for in vitro quantification of soluble analytes in liquid samples. ELISA can be used to detect and/or quantify any analyte that can be bound by an antibody, such as cytokines, CD antigens, apoptosis markers, hormones, metabolites, growth factors, and more.

Mycoplasma – The Silent Enemy

Although mycoplasma is the smallest known prokaryotic organism with a tiny size range of 0.15 to 0.35 µm, it can quickly become a huge problem in cell culture labs!

Product Highlights: Immune Checkpoints

Immune checkpoints regulate when and how the immune system is activated. Their correct regulation and function are critical for maintaining a self-tolerant immune system that knows when to act (i.e. during infections, cancer) and when not to (i.e. to prevent autoimmunity). Checkpoints are maintained by a large group of cell-surface receptors, including stimulatory and inhibitory checkpoint molecules that together fine-tune immune signalling.

Immunohistochemistry Image Analysis – an Image Paints a Thousand Words

In our last article we looked at the workflow for a typical immunohistochemistry (IHC) experiment. Regardless of the tissue type under investigation, the target biomolecules, and the detection method chosen, it is necessary to apply a method to extract and analyse the information held on the stained slides. The detection method used will influence image analysis to some extent i.e. light microscopy or fluorescence microscopy is used to visualise slides after chromogenic or fluorescence detection, respectively. With the right equipment, it is possible to scan entire slides for easier viewing of entire tissue sections, further analysis and to get high-quality images in digital format for use in publications and presentations.

Antibody Validation 101

Many researchers start their hunt for new antibodies by scouring the literature to find out which antibodies others are using for the same target. While this is a reasonable starting point, simply choosing an antibody with reported target specificity and sensitivity is not enough, even if the published data looks very convincing! In practice, the suitability of an antibody for a given sample type, experimental conditions, and application depends on many more parameters than affinity for a target protein.

Sample Collection and Preservation – Critical Starting Points in Your Research!

In the current ‘omics’ era, there seems to be no limit to the number of ways we can investigate our favorite organism or cell type. We can study genomes, transcriptomes, epigenomes, metabolomes, proteomes, kinomes, and more. Parallel advances in flow cytometry and other immunological techniques make it possible for us to simultaneously detect and profile new and characterized cell surface markers in robust multiplex setups.