Science Hub

Molecular Biology

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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Modulate Gene Expression With CRISPRa And CRISPRi

 

If you’re new to CRISPR, then you might think of it as a tool to disrupt (knock out) or add (knock-in) sequences or genes of interest to the genome, but in this post, we will introduce you to two newer CRISPR applications that do something rather different.

Gene Silencing – RNAi or CRISPR?

No matter what area of research you are working in, at some point or another you may need to decipher the function of a completely unknown protein or better understand the mechanisms of a cellular pathway.

There are many ways of going about this, either by interfering at the protein level through the use of small molecule inhibitors or blocking antibodies, the genetic level by altering the gene sequence that encodes your protein of interest, or at the transcriptional level by interfering with the RNA transcript.

This piece looks at CRISPR and RNA interference (RNAi) – two commonly used methods to interrogate gene function – and compares the pros and cons of each approach within a lab research setting.

New NGS Library Prep Kits from Zymo Research!

 

Our supplier Zymo Research recently launched two brand new NGS kits that are designed for fast, normalisation-free, library preparation for 16S rRNA or ITS rRNA sequencing.

CRISPR: History and Applications in Brief

 

CRISPR-Cas9 is a modern genome-editing tool that has its roots in an ancient prokaryotic immune system. Since its first adaption as a genome-editing tool in living eukaryotic cells less than a decade ago, CRISPR has undergone massive development and today it represents a rapidly growing toolbox with broad applications.

This piece gives a brief overview on the history of CRISPR and how it is used today.

Epigenetics Crash Course 4

Welcome back to the 4th and final installment in our epigenetics crash course series!

So far, we’ve introduced the field of epigenetics, and examined bisulfite as well as non-bisulfite approaches to studying nucleic acid methylation. In this last piece, we look at methods to study chromatin and histones.

Epigenetics Crash Course 3

Our Epigenetics series continues! So far in Part 1 and Part 2, we’ve introduced the field of epigenetics and the bisulfite-based approaches to studying DNA and RNA methylation. In Part 3, we look at non-bisulfite methods to study nucleic acid methylation.