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Pathology

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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Immunohistochemistry Image Analysis – an Image Paints a Thousand Words

In our last article we looked at the workflow for a typical immunohistochemistry (IHC) experiment. Regardless of the tissue type under investigation, the target biomolecules, and the detection method chosen, it is necessary to apply a method to extract and analyse the information held on the stained slides. The detection method used will influence image analysis to some extent i.e. light microscopy or fluorescence microscopy is used to visualise slides after chromogenic or fluorescence detection, respectively. With the right equipment, it is possible to scan entire slides for easier viewing of entire tissue sections, further analysis and to get high-quality images in digital format for use in publications and presentations.

The Typical Immunohistochemistry Workflow

Immunohistochemistry (IHC) ties together immunological, biochemical and microscopic techniques to yield visual information about proteins and other macromolecules in tissues. Targets are detected through the use of labelled antibodies that penetrate tissues and specifically bind proteins and other cellular components of interest, e.g., organelles.

Beginner’s Guide to Immunohistochemistry 2: Choosing a Secondary Antibody

Welcome back to our beginner’s guide to immunohistochemistry (IHC). In this post, we’ll follow on from our last article on choosing a primary antibody, bringing you the most important considerations for choosing a secondary antibody.

Beginner’s Guide to Immunohistochemistry 1: Choosing a Primary Antibody

As with any other experimental setup, there are many factors to consider when embarking on a new immunohistochemistry (IHC) project, for example, the kind of tissue type you will stain and whether it will be fixed or frozen, the labelling/detection system, suitable staining controls, and the way in which you will analyse your data, to name a few.