Science Hub

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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RNA Isolation in 7 Minutes – Go!

Total RNA is the starting point for a plethora of applications that can help to answer questions about the mechanisms of human and plant disease, gene regulation, embryonic development, transcriptional responses to treatments and stimuli, and more. Regardless of the application in hand – whether it be cDNA synthesis for reverse-transcription PCR, RNA-seq, northern blotting or otherwise, sufficient amounts of high-quality RNA will be a requisite.

DNA Isolation From Low Input Samples

Whole genome sequencing and related ‘omics’ technologies have paved the way for many new possibilities within basic and clinical research, and they are becoming an integral part of the personalised medicine era by identifying individual genotypes that can direct diagnosis and therapy.

Besides a sequencing instrument, reagents and personnel who can translate the sequencing reads into workable data, the only, albeit, most important prerequisite is a sufficient amount of high-quality genomic DNA.

To Gate or Not to Gate?

Flow cytometry is one of the most intensely used techniques in immunology that creates big amount of data in short amount of time. It is possible to obtain information on cell surface markers, cellular processes such as apoptosis, and signaling pathways and much more.

To get a reliable result in flow cytometry, proper controls for accurate gating is essential. Gating means to sequester specific group of cells. This is generally a manual step performed during and after data acquisition. Even though gating can be straightforward for substantial cell groups such as CD4+ cells in PMBC samples; it can be tricky when it comes to less obvious populations, for cells that have transient marker expression or small cell populations.

In this blogpost, we want to give some tips on how to use flow cytometry controls to get more successful gating and subsequently more consistent data.

Restriction Enzymes 101

Restriction enzymes or restriction endonucleases recognise short, specific DNA sequences known as restriction recognition sites, and make double-stranded cuts at or adjacent to these sites. These enzymes are naturally occurring in bacteria and archaea where they function as an ancient sequence-directed immune system, chopping up phage DNA at restriction sites in the genome of the invading phage.

ELISA 101 Part 2: ELISA Standards

ELISA (Enzyme Linked Immuno-Sorbent Assay) is a highly sensitive plate-based assay for in vitro quantification of soluble analytes in liquid samples. ELISA can be used to detect and/or quantify any analyte that can be bound by an antibody, such as cytokines, CD antigens, apoptosis markers, hormones, metabolites, growth factors, and more.

Size Selection for NGS

In previous posts about next generation sequencing (NGS), we covered the key steps in library preparation (for RNA-seq), top tips for NGS success, and the ins and outs of long-read DNA sequencing. This time we will look more closely at size selection.