Flow cytometry is one of the most intensely used techniques in immunology that creates big amount of data in short amount of time. It is possible to obtain information on cell surface markers, cellular processes such as apoptosis, and signaling pathways and much more.
To get a reliable result in flow cytometry, proper controls for accurate gating is essential. Gating means to sequester specific group of cells. This is generally a manual step performed during and after data acquisition. Even though gating can be straightforward for substantial cell groups such as CD4+ cells in PMBC samples; it can be tricky when it comes to less obvious populations, for cells that have transient marker expression or small cell populations.
In this blogpost, we want to give some tips on how to use flow cytometry controls to get more successful gating and subsequently more consistent data.