Science Hub

Welcome to Nordic BioSite's Life Science Blog. Here, we will provide commentary on current events and trends in biological sciences, interesting stories about important scientists, technical tips and suggestions, and much more. Read on and learn something new.
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Multiplex Real-Time PCR for Beginners

In case the name doesn’t give it away, multiplex real-time PCR involves amplifying multiple DNA or RNA targets simultaneously in a single PCR reaction. This requires the presence of a specific pair of primers and a complementary DNA-binding probe for each target under study.

Size Matters! Long-Read DNA Sequencing

Modern genomics seems to be undergoing a shift from using short-read technologies to sequence large numbers of genomes with the aim of detecting SNPs, to sequencing fewer genomes using long-read technologies that can resolve more complex events, e.g., structural rearrangements, copy number variations, and repeat expansions.

RNA-Seq 5: Data Validation

RNA-seq generates vast amounts of data and when all of this is sorted out and boiled down to results, the researcher is usually left with lists and heat maps showing transcripts that are expressed, upregulated or downregulated across the experimental conditions tested. Exactly how and in what situations this data should be validated is a topic of debate among researchers.

Product Highlights: Immune Checkpoints

Immune checkpoints regulate when and how the immune system is activated. Their correct regulation and function are critical for maintaining a self-tolerant immune system that knows when to act (i.e. during infections, cancer) and when not to (i.e. to prevent autoimmunity). Checkpoints are maintained by a large group of cell-surface receptors, including stimulatory and inhibitory checkpoint molecules that together fine-tune immune signalling.

Immunohistochemistry Image Analysis – an Image Paints a Thousand Words

In our last article we looked at the workflow for a typical immunohistochemistry (IHC) experiment. Regardless of the tissue type under investigation, the target biomolecules, and the detection method chosen, it is necessary to apply a method to extract and analyse the information held on the stained slides. The detection method used will influence image analysis to some extent i.e. light microscopy or fluorescence microscopy is used to visualise slides after chromogenic or fluorescence detection, respectively. With the right equipment, it is possible to scan entire slides for easier viewing of entire tissue sections, further analysis and to get high-quality images in digital format for use in publications and presentations.

RNA-Seq 4: Planning Your Experiment

Welcome back to the RNA-seq series! In this article, we will go through some of the main parameters you will need to consider when setting up a new RNA-seq experiment!